Sentence examples for direct label from inspiring English sources

Fluorescently labeled cDNAs were generated from 20 μg of total RNA in each reaction using the Agilent Fluorescent Direct Label Kit and 1.0 mM Cyanine 3- or 5-labeled dCTP (PerkinElmer, Boston, MA), and following user's manual.

Twenty micrograms of total RNA were used to generate fluorescent Cy-3-labeled cRNA (control cells) and Cy-5-labeled cRNA (treated cells) using an Agilent Fluorescent Direct Label Kit.

Per microarray, 20 μg mRNA, pooled from 2 livers, was reverse transcribed with Cy3-labelled dCTP (Perkin Elmer, Boston, USA), using the Agilent Fluorescent Direct Label Kit.

500 ng of mRNA from tumors with high COX-2 patients was labeled with Cy-3-dCTP (Amersham BioSciences) in a cDNA synthesis reaction with Agilent Flourescent Direct Label.

The probe bears a direct label (e.g. fluorescein) or a tag for subsequent detection (e.g. biotin).

Total RNA (20 μg) was labeled using a Fluorescent Direct Label Kit (Agilent Technologies) and simultaneously reverse transcribed into cDNA.

cDNA fluorescence-labeled probes were obtained by the direct labeling procedure in the presence of modified cy-3 and cy5 dCTP (Perkin Elmer) [51].

Devices may be used as a transducer for direct label-free biosensor development.

This constitutes a direct, label-free and signal-on electrochemical immunosensor, with a very low detection limit of ca. 1 pM, i.e. 0.2 ng L−1, one of the lowest reported for such immunosensors.

A direct, label-free immunosensor was designed for the rapid detection and quantification of staphylococcal enterotoxin A (SEA) in buffered solutions using quartz crystal microbalance with dissipation (QCM-D) as transduction method.

Both the drug loaded and unloaded formulations were labeled with 99mTc by the direct labeling method.