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Caliban's language is not learnt-up eloquence but the direct expression of a richly perceiving mind, and it rebuffs all external judgment of him.
TERRY LAVIN, Chicago Kimball's own cooking may not be creative, but the very reason we crave Mom's soup, Grandma's pie or Dad's salad dressing is that each dish is a direct expression of a person's touch, taste and interpretation of the food they have prepared.
Weisberg, a representational artist, posits that "Post-postmodernism is going to be more like the great periods of classical and Renaissance art, and therefore much more about the direct expression of a sensuous experience of the world with a deep and abiding connection to human aspirations".
In this interaction, MIARS coding can capture the patient cue (MIARS Level II – direct expression of a concern) and the provider's acknowledgment of the patient's feelings.
The ability of the pSM30 system to direct expression of a mature miRNA was confirmed by qRT PCR as shown in Figure 2. Transfection with pmiR-212 increased expression of mature miR-212 in HEK-293 and in HeLa cells.
A 1.9 Kb DNA fragment covering the intronic sequences upstream of the miRNA cluster and spanning the next upstream exon proved sufficient to direct expression of a luciferase reporter gene in S2 cells (Fig. 3C; supplementary material Fig. S2A).
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Free love advocates sometimes traced their roots back to Josiah Warren and to experimental communities, viewed sexual freedom as a clear, direct expression of an individual's sovereignty.
That study employed direct expression of an attenuated diphtheria toxin from the melanopsin locus, which caused slow degeneration of the mRGCs over several months, as assessed by the loss of a Tau LacZ transgene expression or melanopsin immunostaining.
These vaccines contain the alphavirus nonstructural genes, the 5' and 3' cis-active replication sequences and the subgenomic promoter that directs expression of a heterologous gene [44].
To test whether this feature also applies to the cold phenotypes, we directed expression of a FLAG tagged form of Cold in the tracheal cells of a cold mutant embryo, using the btlGAL4 driver.
The EBP1 fragment we isolated lacks a TATA box, and exhibits promoter activity, as it is capable of directing expression of a luciferase reporter transfected into neonatal epidermal human keratinocytes (Fig. 1C), and is activated about 10-fold by exogenous expression of E2F1, −3 and −5 in these cells (Fig. 1C).
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