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LE agarose was used to immobilize cells to aid with direct counting (Liu et al. 2001).
This was based on colony forming units in PII-end compost extracts and by direct counting.
(PDF 110 KB) 13568_2012_51_MOESM2_ESM.PDF Additional file 2: Example of bac/light direct counting results.
Both Quantcast and Google (Analytics) offer direct counting of pageviews (but even these methods can be abused).
This method involved direct counting of viable cells and hence eliminated the possibility of interference from occurring.
After detachment, 10 mL of sample was immediately fixed with 2% formaldehyde (final concentration) and cooled at 4 °C for later direct counting of cells.
Data integrity was first checked by comparing the total radioactivity in the brain determined in two independent ways: ● FE: direct counting of microspheres in all brain sections.
The number of doorway modes in the regions of 200 to 700 cm−1 was evaluated by the direct counting method.
The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks.
The most accurate method for quantifying viral load was direct counting, followed by thin section of pelleted supernatant and negative stain after sucrose concentration.
It has been calibrated using direct counting with a NaI detector, and its efficiency has been shown to be well described by a GEANT4 simulation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com