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When only direct categories are considered, compounds belong on average to 13.5 MoA categories.
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It is likely to arise from characteristics of the upstream regions of the direct category genes.
The top ten sequences are listed in Table 5 for the direct category and Table 7 for the indirect category.
The occurrence of heteropolymeric AT and TA dinucleotides is unusually high in the direct category compared to the indirect category.
The same is true for the direct category sequences in Table 5, with an average ratio of 4.2.
The completely A/T heteropolymeric sequences ATATA, TATAT, ATATAT, and TATATA occur at high rates in the direct category.
Table 4 shows the top ten 5-mer and 6-mer sequences from the direct category according to this criterion.
Another observation is that the A+T content levels of individual members of the direct category lists are uniformly high.
These observations support the conclusion that A/T richness is a characteristic of the direct category sequences much more than it is of the indirect category sequences.
The difference and ratio criteria for choosing overrepresented sequences yield more similar results for the direct category than for the indirect category.
Since the first two of these causes are not expected to be different for the direct and indirect categories, the increased level of shared sequences for the direct category is telling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com