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The established healthy control CoQ10 concentration range in the literature is 1.04 μmol/L ± 0.33, though we measured a set of healthy controls in this study to provide direct comparison and validate our methods [ 20].
Moreover, by providing force-dependent dissociation rates this method allows direct testing and validating of any energy landscape model.
Luciferase reporter assays in five GC cell lines indicated their direct binding and validated by western blotting.
In conclusion, the sensitivity of MRI for ILC is higher than that achieved by any other modality, in direct comparison and validated by literature, and is equal to the overall sensitivity of MRI for malignant lesions of the breast.
Further experiments will be needed to confirm that these genes are direct target genes and validate these targets in other cell systems, particularly lymphocytes.
Well standardized and validated direct and indirect measures of efficacy and noncognitive outcomes are also a crucial issue.
In silico database analysis and luciferase reporter assay were applied to predict and validate the direct target, respectively.
Error analysis and numerical comparisons with direct evaluation validate the efficiency and accuracy of the algorithm.
A matching Kd of ∼5 mM was obtained for 6 under both direct and competitive conditions, thereby validating the approach.
The disconnected components and singletons in the network of experimentally validated direct and indirect interactions, as well as GAP's novel predictions are provided by Additional file 1: Figure S5.
Using common performance evaluation measures and a gold-standard database of experimentally validated direct and indirect interactions, we have shown that GAP significantly outperforms the existing interaction prediction tools in correctly identifying known interactions.
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