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The designed strategies comprised nested as well as direct amplification of environmental DNA.
Nuclear ribosomal DNA sequences characteristic of Sebacinaceae were detected by direct amplification of DNA from field collected ectomycorrhizal samples.
The efficacy of direct amplification of DNA from dilute bloodstains, saliva stains, and touch samples was evaluated using microFLOQ® Direct swabs and the GlobalFiler™ Express system.
LATE-PCR is an advanced form of asymmetric PCR which results in direct amplification of large amount of single-stranded DNA.
Result were also compared with direct amplification of cells spiked into distilled, tap, and cooling water samples as well as extracted DNA by qPCR.
The applicability and sensitivity of MGE-PCR in epidemiological studies was demonstrated by direct amplification of T. gondii from sheep tissue samples.
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An immune response based on directed amplification of specific receptors for antigen produced through a somatic diversification process that includes somatic recombination of germline gene segments encoding immunoglobulin superfamily domains, and allowing for enhanced responses upon subsequent exposures to the same antigen (immunological memory).
In this report we implemented a simple procedure based on a modification of the Rapid Amplification of 5' complementary DNA ends (5' RACE [20], [21]) named Directed Amplification of TSSs (DMTSS), and a high throughput pyrosequencing strategy (HTPS) with Roche's 454 GS20 instrument, to experimentally determine as many TSSs as possible in the E. coli K-12 genome.
DAMD – Directed Amplification of Minisatellite DNA, NJ – Neighbor Joining; RAPD – Random Amplified Polymorphic DNA.
Based on PCR in silico anchor results, we found that 11 BACs were anchored on both A- and D-genome homoelogous chromosomes; 2,111 primer pairs amplified in silico DNA sequences from four cotton species, with 762 primer pairs directing amplification of same-sized DNA fragments, and 1,349 primer pairs giving PCR products with different predicted sizes.
Reamplifications of PCR products when necessary were done either by direct amplification from of an aliquot of the first PCR or subsequent to gel extraction of the primary amplification band using a Qiagen Gel Extraction Kit.
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