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Following 3 washes, each plate was incubated with culture supernatants and a series of dilution standards for 2 h.
Absolute quantification was carried out in the presence of dilution standards for each gene.
The standard curve of HULC expression was plotted using the results of the serial dilution standards.
In each assay, fully methylated, WGA DNA (unmethylated), different DNA methylation percentage dilution standards and no template controls were also performed.
In each assay, fully methylated, peripheral blood DNA (unmethylated), different methylation percentage dilution standards and nontemplate controls were included as controls and standards.
The TTO activity against the C. albicans strains tested was determined by broth macrodilution using the general dilution standards as described by PN-EN ISO 20776-1 2007 [ 24].
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An eight dilution standard curve was then established (1 40 through 1 2560) and unknown samples diluted 1 40 were tested side-by-side with the standard curve on each plate.
An optimized method was developed to analyze environmental soil and sediment samples for 237Np, 239Pu, and 240Pu by ICP-MS using a 242Pu isotope dilution standard.
New results of a systematic study of partial molar volumes at infinite dilution (standard partial molar volumes) in water of several hydroxyderivatives of aliphatic hydrocarbons are summarized.
A five-fold log-scale dilution standard curve was generated for RCP1 using RPC1 cRNA.
A 10 fold serial dilution standard curve was included for each gene of interest and both normalisers on each plate.
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