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The MLR model is trained based on a data bank of 345 experimental binary diffusivities of hydrocarbons in infinite dilution solutions.
Working dilution solutions of TRAM34 were made up on the day by slowly mixing the stock solution with physiological saline that had been gassed with 95% O2-5% CO2 for at least 30 minutes [31].
Biofilm was stained for 5 min with 1 : 1000 dilution solutions (in 0.9% NaCl) of SYTO9 for live organisms and with propidium iodide for dead organisms.
The control column only contained 100 μL of the positive control (ampicillin), negative control (water), and extraction dilution solutions (i.e., identical to the experimental column except no bacteria were present).
In brief, 1 mL of different peptides dilution solutions was poured into a series of sterile plates, and then 9 mL appropriate medium that was preincubated at 37°C was added into a plate and was mixed gently to reach various concentrations (512, 256, 128, 64, 32, 16, 8, 4, 2, 1, and 0.5 μ g/mL).
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Serum samples were diluted in a dilution solution containing 0.9% NaCl solution, 0.1% (wt/vol) Fetal Calf Serum and 0.2 M 2-mercaptoethanol.
Following adsorption, the antibodies were diluted 1 500 in antibody dilution solution and stored at 4°C.
The blank standard was taken to be the dilution solution of MeCN MeOH (30 70) with no added drug.
The results show certain patterns of protein adsorption, which depend to a large extent on the dilution solution and filter material, and to a much lower extent on the proteins' biophysical properties.
Warming procedures were performed by placing the Cryotop in a warming solution (TS, 1 mol/L sucrose) for 50 60 s at 37°C and moving into a dilution solution (DS, 0.5 mol/L sucrose) for 3 min at room temperature.
This blocking solution also served as the dilution solution for all involved antibodies.
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