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Serum samples were diluted in a dilution solution containing 0.9% NaCl solution, 0.1% (wt/vol) Fetal Calf Serum and 0.2 M 2-mercaptoethanol.
Following adsorption, the antibodies were diluted 1 500 in antibody dilution solution and stored at 4°C.
Slides were washed in PBS, blocked in 10% normal horse serum (NHS) in PBS for 30 min and incubated with polyclonal rabbit anti-Nav1.8 antibody (Chemicon, CA, USA) diluted 1 200 in antibody dilution solution (10% NHS and 0.4% sodium azide in PBS) overnight.
The blank standard was taken to be the dilution solution of MeCN MeOH (30 70) with no added drug.
The results show certain patterns of protein adsorption, which depend to a large extent on the dilution solution and filter material, and to a much lower extent on the proteins' biophysical properties.
The meristems were then rapidly rewarmed by dilution solution, transferred to regeneration medium and stored in the dark for two weeks at 20 °C, and then cultured under white fluorescent light at an intensity of 2000 lux, with a 16 h photoperiod at 20 °C.
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The MLR model is trained based on a data bank of 345 experimental binary diffusivities of hydrocarbons in infinite dilution solutions.
Working dilution solutions of TRAM34 were made up on the day by slowly mixing the stock solution with physiological saline that had been gassed with 95% O2-5% CO2 for at least 30 minutes [31].
Biofilm was stained for 5 min with 1 : 1000 dilution solutions (in 0.9% NaCl) of SYTO9 for live organisms and with propidium iodide for dead organisms.
The control column only contained 100 μL of the positive control (ampicillin), negative control (water), and extraction dilution solutions (i.e., identical to the experimental column except no bacteria were present).
Final drug concentrations were achieved by dilution with solution A for electrophysiological experiments and solution H for experiments in single cells.
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