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Our drug sensitivity database consists of 639 human cancer cell lines in combination with 130 targeted therapy or cytotoxic drugs, assayed in a 9-point 256-fold serial dilution setting.
Repeat studies indicated smoke penetration was very consistent in the RM20S, so smoke delivered to the chamber will be consistent at this dilution setting of 1 60 (smoke:air, v/v).
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The third most accurate quantification method was different for each dilution set, indicating how variable the predictions made by these methods can be.
The amplification efficiency of each primer pair was determined using a serial dilution set made from a pool of cDNA samples.
For two of the serial dilution sets (gravimetric dilution series 2 and 3), eight replicate ddPCRs were prepared from a single eight-channel droplet generator cartridge for each of the seven solutions.
* PCR efficiency defined as the slope of the Log-linear phase of a dilution set as described in Figure 3A For an accurate quantification it is also necessary to verify that PCR efficiency is independent of the initial amount of target DNA.
This dilution set was very close to the curve-derived maximal sensitivity regarding the tests' cut-off criteria of ∼2.5×103 mg−/mg+.
Figure 2 illustrates how the average Ct value alters across the three days (which have three different dilution sets) for each plasmid copy number.
To quantify transcript levels, a standard curve was run in parallel reactions and employed a serial dilution set of p40 encoding template DNAs.
The dilution rate (setting the generation time) was set to four hours during the first month and two hours thereafter.
Serial dilutions of standard ranging from 1∶5 dilutions (5 fold dilutions) were set up for beta actin control and KCNMA1 target in separate duplicate wells.
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