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We also constructed a dilution scale, starting with the sample taken at diagnosis and serially diluting it 10-fold five times.
Results, summarized in Table 3, showed that IgG-IgA cross-reactivity was uniquely observed at high concentration of standard antibodies (i.e. at the first two dilution points of the dilution scale).
These data exhibit a sigmoidal shape when plotted on an OD-log10 dilution scale.
For each gene analysed, a cDNA dilution scale from 5 to 50000 times was performed in order to assess efficiency of PCR.
You don't need a refractometer to explore the power of dilution, though a scale is advisable for getting close to Mr. Hoffmann's sweet spot for coffee, since volume measures are unreliable and the decimals count.
A five-fold log-scale dilution standard curve was generated for RCP1 using RPC1 cRNA.
A five-fold log-scale dilution standard curve was generated for each gene using D. melanogaster genomic DNA in order to perform absolute quantification for each sample.
The accuracy of this CO estimate is achieved by comparing (calibrating) the PulseCO with a known CO, for example from the lithium dilution or LiDCO which scales the software algorithm and reduces the bias to the actual CO [ 1].
The modeling results revealed a tremendous water flux decline (10 → 0.82 LMH) and short solids retention time (SRT: 5 days) due to salt accumulation and DS dilution when OMBR is scaled up using commercially available DS and FO membrane.
The endpoint titre data were converted to dilution data from the exponential scale (titre) to a linear scale (dilution) by taking the logarithm of the endpoint titre, that is, log2 titre)=dilution.
In calculating actual concentrations, apparent concentrations were scaled based on dilution or concentration in GC injection preparation.
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