PCR amplicons were prepared for cycle sequencing by diluting them with water using a dilution range of 1 3 to 1 6, depending on the reaction yield, as determined by agarose gel electrophoresis.
The membranes were incubated overnight at 4°C with rabbit polyclonal antibody, targeting SOD1 (Santa Cruz Biotechnology, CA, USA) (dilution range 1 400), SOD2 (Santa Cruz Biotechnology) (dilution range 1 400), SOD3 (Santa Cruz Biotechnology) (dilution range 1 750), iNOS (Santa Cruz Biotechnology) (dilution range 1 400) or anti-β-actin 1 2000, in the presence of 5% milk.
The assay was linear over a 106 dilution range of template concentrations.
More information about post-treatment details such as vortex intensity, dilution range, or spread volume were rarely given.
Although nicotine and carbonyl concentrations were linear over the tested dilution range, particle mass deposition increased nonlinearly.
For the e-cigarette, deposited particle mass in the same dilution range was 0.34 3.34 µg/cm2/puff [Fig. 6 (top); Table 5].
The subsequent dilution range before spreading the sample on agar is dependent on the plasma treatment severity and the initial cell concentration.
Anti-rabbit immunoglobulin G (IgG) peroxidase-linked secondary antibody was incubated with the membranes for 1 additional hour (1 10000 dilution range).
Membranes were incubated overnight at 4 °C with rabbit polyclonal antibodies against SOD1 (1 400), SOD2 (dilution range, 1 400), SOD3 (dilution range, 1 750), iNOS (1 750), eNOS (1 750), pVASP (1 600) or β-actin (1 2000) and then washed with TTBS.
The QCM quantified the mass range of delivery of whole smoke particulate from 3R4F reference cigarettes as 25.75 ±2.30 μg/cm2 to 0.22 ±0.03 μg/cm2 (within the standard dilution range of 1 5 1 400 (v:v)), and for 1 mg commercial cigarettes as 1.42 ±0.26 μg/cm2 to 0.13 ±0.02 μg/cm2 (dilution range 1 5 1 100 (v:v)).
The dilutor syringes work independently, with the capability of delivering an extensive smoke dilution range (1 2 -1:4,000 [smoke:air, v/v]) and delivering up to eight different doses of whole smoke simultaneously.
