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However, we feel that our dilution procedure (whereby a gametocyte sample is diluted 5 times and each generates a TTP) provides a strong validation procedure within the model.
Biochemistry tests for the determination of solute concentrations were performed after diluting 1 part concentrate with 34 parts sterile water to simulate the dilution procedure within the dialysis machine.
To assess self-renewal, single cells were harvested by limiting dilution procedure and wells were scored once a week.
Further, the dilution procedure in Step-1 label the test tubes as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10.
The CVM combined with this new one-step warming in-straw CP dilution procedure could be used for direct ET under field conditions.
Finally, IVP blastocysts were vitrified using conventional fibreplugs to maintain a high embryo recovery rate, and then warmed using the one-step warming in-straw CP dilution procedure, but using an adapter with a wider opening coupled to the French straw and a heated metal chamber to protect and keep the straw at 41 °C (experiment 4).
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The cells were resuspended in fresh medium, diluted to 10 cells/ml using limiting dilution procedures, seeded into 96-well plates (0.1 ml/well, contained about one cell), and cultured in DMEM medium containing 10% FBS.
Furthermore, dilution procedures altered the matrixes of the sample and prompted to have more compatibility with further analyzing.
Dilution procedures have followed standard methodology described at the Brazilian Homeopathic Pharmacopoeia.
We then selected several individual cell clones via limiting dilution procedures.
ELISA has limitations, like the need for a large sample volume, a narrow dynamic range, and complicated dilution procedures [ 18, 19].
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