Sentence examples for dilution points from inspiring English sources

PCR efficiency was evaluated by using a standard curve of five serial dilution points.

The standard curve was generated using a tenfold dilution series over three dilution points that were measured in triplicate.

From the 11 RT enzymes studied, five enzymes (ACC, HIV, SS3, MML and TSR) produced reproducible results across all dilution points tested.

The RT enzymes that produced erroneous efficiencies due to non-specificity (eg SML) or failed altogether at the low copy end were excluded from further analysis that required all dilution points.

The assay included a no template control, a standard curve of four serial dilution points (in steps of 10-fold) of a standard cDNA, and each of the tested cDNA populations.

The 5 enzymes exhibited clear linear relationships among the dilution points and Cq values obtained from amplification of Armored HIV RNA, with each yielding an Rsq of ≥0.95, except for the HIV RT enzyme (0.85).

The Cqs of each standard dilution point across replicates were generally similar although SS3 and ACC RT enzymes were the most consistent in their amplification of low copy number templates (Figure 1).

The slope and correlation coefficient of each standard curve were calculated based on the median threshold cycle (CT) values measured for replicates of each dilution point ranging from 106 to 101 DNA templates.

1D and 1E) were either plated or quantified by qPhage, each 10-fold dilution point resulted on average in a proportional variation of 7-fold for TU-counting and 9.3-fold for qPhage, with an SEM of the triplicates at all dilutions of 32.5% for TU-counting, versus 7.8% for qPhage.

For each of the four qPCR runs, each dilution point was assayed in triplicate.

The RSDr and r-values of the Cq-values were calculated at each dilution point.