Sentence examples for dilution of the two from inspiring English sources
Serial dilution of the two strongest MM samples (between 1 and 30 μg) demonstrated that at protein loads of greater than 10 μg per well, the densitometry values reached a plateau (data not shown).
Technological advances, Tuesday's ruling said, altered neither the applicable legal standards nor how much dilution of the "one person, one vote" principle is tolerable.
A template mix including ten-fold serial dilution of the four in vitro transcribed virus targets was tested.
Blots incubated with a 1 20 dilution of the four sera were allowed to hybridize overnight at 4°C.
The results of the current study are consistent with the hypothesis that solids stored in sewers between events are mobilized during stormflow, resulting in the observed lack of dilution of the four compounds with high log Kow values during CSO discharges.
The semiquantitative nature of this protocol was validated by densitometric analysis of serial dilutions of the two samples with the strongest bands.
The quantitative variation results of signal intensities in Figure 8b are in complete accordance with the image visualization in relation to in vitro dilutions of the three materials.
Subsequently, cells were rinsed three times in PBST, incubated for 1 h at room temperature in the dark with 1∶1000 dilutions of the three AlexFluor secondary antibodies, ALexaFLuor405 (goat antimouse), AlexaFluor488 (donkey anti-rabbit) and AlexaFluor546 (goat anti-rat) (Molecular Probes, Invitrogen) for EGFR, Src and Stat3 detection, respectively.
Ten-fold serial dilutions of the fourteen mAbs were incubated with botulinum toxin type A1 containing approximately 10 mouse 50% lethal doses (MLD50/ml) (Table S2), in 50 mM phosphate buffer pH 6.5 containing 1% gelatin (PB-G), for 30 min at room temperature.
In contrast, the masked dilutions of the three positive control reagents performed as expected.
The template mixture of ten-fold serial dilutions of the four in vitro transcribed virus targets was tested in triplicate by mRT-qPCR assay.
