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Exact(19)
The mixture was mixed well, and the clotting time T (s), the time period starting from the addition of test material to the first appearance of clots of milk solution, was recorded and the clotting activity was calculated using the following formula: SU = 2400 × 5 × D/T × 0.5; T = clotting time (s); D = Dilution of test material.
Growth inhibition was determined by serial 2-fold dilution of test extract or compounds in 96-well microtiter plates to determine Minimal Inhibitory Concentrations (MICs).
Agglutination titers were defined as the highest dilution of test antibodies that caused greater than three times the number of aggregates seen in the same dilution of NMS or control antibodies.
The minimum dilution of test serum was 1/50.
The initial dilution of test sera was 1 101.
A 1 100 dilution of test serum was added to the wells.
Similar(41)
The dashed line represents the lowest dilution of tested sera.
CFU/ml counts were determined by the serial dilution of test-cultures onto TSA, followed by enumeration after overnight growth.
Different dilutions of test sample extracts, positive control, and 100%% extracts of negative control in triplicate were placed on a subconfluent monolayer of L-929 cells.
Dilutions of test and standard compounds were prepared in double strength nutrient broth for bacterial strains and sabouraud dextrose broth for fungal strains [18].
Briefly, duplicate plates (96 well) containing test samples were prepared; one containing two-fold dilutions of test compounds and the other containing test agents plus 0.8 M sorbitol as an osmoprotectant.
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