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Exact(15)
Standard curves constructed from serial dilution of standard plasmids were analyzed.
The working concentrations were prepared by approximate dilution of standard drug solution.
Calibration curves, prepared by dilution of standard stock solutions with hexane, were obtained at concentrations between 0.005 0.1 mg L−1 and using anthracene d10 (1.14 mg L−1) and perylene d12 (1.05 mg L−1) as internal standards.
Sodium arsenite (NaAsO2) was used for the preparation of standard As III) solution, and the required As III) concentrations for experiments were prepared by serial dilution of standard solution on daily basis.
In this assay, the primers were designed and custom-synthesized based on nucleotide sequence of VP2 gene of CPV 2. A standard curve was plotted using 10-fold serial dilution of standard plasmid DNA and Ct value.
Limits of detection (LODs) and limits of quantitation (LOQs) were determined by serial dilution of standard solutions.
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Standards of selected metals were set by dilution of stock standards which were prepared using analytical grade salts of metals (Merck Multielement standard 1000 mg/L).
Background genomic DNA from HPV-negative cell line C33a was used as negative control and for dilution of standards as prescribed by WHO.
Ranges for each analyte assay were evaluated by dilution of standards to determine upper ranges where high-end hook effect and apparent antibody saturation are avoided and lower ranges that are above detection limits.
Ten-fold dilutions of standard DNA ranged from 1 × 108 to 1 × 101 copies/µL.
Serial dilutions of standard stock solutions of anions were prepared, and the real resistance from the fit of Nyquist plots were determined at 25.0 °C.
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