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Bleach-sedimentation involved a 10-fold dilution of specimens and the decrease in the number of acid-fast bacilli visible was significantly less than 10-fold (p = 0.001).
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Serial dilutions of specimens were cultured with gentamicin (which allowed growth of EF3030 and both mutants but reduced growth of oral commensal organisms) and with gemifloxacin (which allowed growth of only Phe/Phe and Phe/Tyr).
The starting dilution of serum specimens was 1 25 (5 ).
The starting dilution of serum specimens was 1 25 for the in-house IFA and 1 10 for the EUROIMMUN kit.
Briefly, the samples were re-tested using the Vironostika HIV-1 Microelisa System (BioMerieux, Raleigh, NC, USA) EIA, which employs testing of a 1∶20,000 dilution of the specimen under modified incubation conditions.
Yet, 17 samples became positive after prior 1/10 dilution of the specimen in PCR buffer.
A serial dilution of the specimen cultured onto coverslips in 24 well plates with 20 hr growth 2 × 10 HEp-2 cells to determine the viable infectious yield [ 46].
An antigen titer was calculated from the thermal contrast by using a calibration curve established by 2-fold serial dilutions of 3 specimens in triplicate with known CRAG LFA titers (R = 0.97).
Multivariable urinary mercury models adjusted for urinary creatinine to account for dilution of spot urine specimens.
It is worth to mention that the high sensitivity of the assay (LOD was 40 pg/ml) allowed the high dilution of a clinical specimen (19 ng/ml after 40 mg dosing) to attain the ROS concentrations in the working range of the assay.
Then 100 µl of a 10-fold dilution of the serum specimen, a blank, a positive and a negative reference serum sample were each added to a separate well.
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