Sentence examples for dilution of specific from inspiring English sources

Membranes were washed with TBST three times each for 10 min and then incubated with an appropriate dilution of specific primary antibodies in TBST overnight at 4 °C.

Besides, when carrier-bound enzymes are used a significant dilution of specific activity is observed since between 90-99% of the percentage in mass of the immobilized enzyme is carrier (Cao et al. 2003).

Membranes were washed with TBST three times for 10 min and incubated with an appropriate dilution of specific primary antibodies in TBST overnight at 4°C.

It has been suggested that it is the dilution of specific food constituents, i.e. yeast extract, that determines the life span of the fruit fly [ 2], as opposed to a reduction of total calories.

We can not fully exclude that dilution of specific cell types in the heterogeneous cell population of mucosal scrapings has lead to undetectable levels of some expected genes, e.g. mucin genes.

Each PCR included 5.25  μl of a 1 : 25 dilution of specific cDNA in water, 1  μl of the specific miRNA Assay Mix and 6.25  μl of 2 × Taq Man Universal PCR Master Mix.

Proteins resolved by SDS-PAGE were transferred to nitrocellulose membrane, which were immunoblotted with optimal dilutions of specific Abs followed by the appropriate anti-IgG-peroxidase-conjugate.

Briefly, for immunoprecipitation analysis, cell lysates containing equal amounts of total proteins (∼2 mg) were incubated overnight at 4°C with the appropriate dilutions of specific antibodies and a mixture of protein A- and protein G-Sepharose beads.

For all assays a standard curve was generated by amplifying serial dilutions of specific PCR products.

Reactions were done in duplicate, and standard curves were calculated from serial dilutions of specific amplified PCR fragments.

Standard curves relating amplification cycle with initial template quantity were generated using serial dilutions of specific RT-PCR products for each gene (obtained from brain cDNA using the same primers) and included in all quantification plates, with qPCR efficiency ranging between 85 97% with R > 0.98.