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Exact(29)
Endpoint titers were defined as the highest reciprocal dilution of sample giving a fluorescence value 3-fold over an equally diluted naïve sample from the same animal.
They were achieved by serial dilution of sample solution using the described UFLC-MS/MS conditions.
A hundred-fold dilution of sample DNA was used as template in the sample qPCRs.
Data are expressed as the OD450 nm reading obtained at the indicated serial dilution of sample.
Titers are expressed as the reciprocal of the highest dilution of sample that inhibited 4 hemagglutinating units (HAU) of virus.
A series of 10-fold dilutions of cDNA was used to generate a standard curve and a 1∶50 dilution of sample cDNA quantified, relative to this series.
Similar(31)
The number of CFUs was obtained by platting 10 μl of a 108 dilution of samples taken at selected optical densities.
Future studies involving Biolog EcoPlates and MicroPlates should take this effect into account, and the dilution of samples is strongly recommended to diminish the "calcium effect".
Distilled-deionized water was used for washing, dilution of samples and preparation of standards.
Filtration and dilution of samples was done respecting ISO 11466/1999 (EU rules).
Enumeration of colony-forming units (CFU) was done through serial dilution of samples in PBS and plating on THA.
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