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Killing of targets was tested at various responder to target ratios and after killing of control syngeneic targets was subtracted, donor specific killing was expressed as lytic units calculated as the dilution of responders giving 20% killing of targets.
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Reactions were stopped with water and plates air-dried and counted using an automated plate reader (Cellular Technology Ltd .. ELISPOT data are reported as means of duplicate measurements, calculated from the linear part of a dilution curve comparing spot-forming units (SFU) per well to serial dilutions of responder cells.
Dilutions of responder cells in complete medium were cultured in triplicate with or without 10 μ M peptide epitope for 40 h.
"She wasn't the nicest of responders," Ms. Vinas said.
However, there are distinct groups of responders and non-responders to Kampo medicines.
The definition of responders varied between studies.
The number of responders was calculated.
The proportion of responders was compared.
Thirty-eight percent of responders were significantly improved compared to 50%% of non-responders.
Nevertheless, when comparing optimal responders with rest of responders we found no differences.
Indomethacin has a the highest odds of responders and complete responders (all p < 0.001).
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