Sentence examples for dilution of reference from inspiring English sources

The same dilution of reference material was added in duplicate to detect adh1, hmg and the GMO gene.

A serial dilution of reference cDNA was also amplified for use in standard curve construction and as a calibrator for subsequent real-time experiments using Stratagene's qRT-PCR Human Reference Total RNA (100 ng).

For both PCR amplifications, the conditions were 95°C incubation for 10 min followed by 35 cycles of 95°C 15 s, and 58°C 1 min. Standard curves were produced by the serial dilution of reference DNAs from 100 to 3.125 ng.

Standard curves were generated from assays made with serial dilutions of reference cDNA to calculate PCR efficiencies (100% +/−15% with r2 > = 0.997).

All primers were HPLC purified, and optimal concentrations were established using serial dilutions of reference cDNA in preliminary experiments.

In the present study, both hsp65 Nested PCR with DNA from reference strains as in sputum sample spiked with serial dilutions of reference strains showed reproducibility.

RNA was extracted from clinical samples (including nasopharyngeal/conjunctival swabs, urine, and tissues) and dilutions of reference strains with the QIAamp viral RNA kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.

To measure gene expression, we used the ΔΔCt method for relative quantification or a standard curve generated from serial dilutions of reference cDNA from pooled BC cell lines for absolute quantification.

Results: To establish the sensitivity of this novel assay a serial dilution of HCV reference standard was used.

The system offers volumetric activities in Bq/m3 using the marine efficiency calibration, which is measured by dilution of three reference sources (99mTc, 137Cs, and 40K) in a special tank.

The assay allowed detection limit of 1 ng of mycobacterial DNA by using serial dilution of all reference strains DNA.