Saturated overnight cultures were diluted 1 100 into saline followed by a 1 100 dilution into the medium used for the assay and grown overnight again.
But rather than suffer the expected dilution into insignificance, Scotland became proportionately the most significant player in the union's empire.
Other products can be measured by dilution into this range.
The physical stability of an immunoglobulin G4 monoclonal antibody (mAb) upon dilution into intravenous (i.v).v
Then the bacterial solution was adjusted by dilution into phosphate buffer to obtain ca. 5 × 105 viable cells/ml.
These results are satisfactory since they ensure human and plant safety after dilution into the ambient air of the greenhouse.
The inactive nematodes were transferred separately from each dilution into sterile distilled water and kept overnight to check whether mortality was permanent or temporary.
All the coated MNPs was sterilized with filter-film (pore size, 0.22 μm) and sonicated before dilution into DMEM culture medium to ensure even particle suspension.
Aβ was rendered monomeric and denatured by dissolution in 8 M urea, pH 10. "Refolding" and fibrillization were initiated by rapid dilution into phosphate-buffered saline, pH 7.4.
Cells were passaged at 1∶10 dilution into selective medium 72 hours post transfection.
Preparing a saturated pre-culture in LB broth followed by dilution into 7H9 also resulted in no growth.
