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These curves were generated using dilution coefficient versus the mean threshold cycle (Ct).
In serial dilution plating the precision of the CFUml-1 estimate is lower, the higher the dilution coefficient.
ODM: Oesophageal Doppler monitor; PPV: Pulse pressure variation; SVV: Stroke volume variation; LiDCO: Lithium dilution coefficient; PAC: Pulmonary artery catheter; SvO2: Venous oxygen saturations; PVI: Pleth variability index.
The overall estimate of CFUml-1 in each experimental unit per sampling time was counted as a weighted mean of all platings per sample where the plating dilution coefficient was used as the weight.
We used weighted mean instead of arithmetic mean to reduce the overestimation error, which could result from plating where number of colony forming units is low and dilution coefficient high [ 44].
However, it must be stressed that all the model predictions were calculated for a single cell, so the estimates of the positive trends for the starch flux and content may arise from the fact that no dilution coefficient term was included in the model.
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The present study offers a multi-step approach, based on pollutant emission factors and atmospheric dilution coefficients, for a local comparative analysis.
Combined with the known dilution coefficients (from the original experimental deep well plates), we calculated bacterial cell counts at each time point for each reporter strain.
The properties needed to make such predictions for any chemical are its vapor pressure, its infinite dilution activity coefficient in water (or their product, the Henry's law constant), and its octanol water partition coefficient.
The UNIFAC model based on the infinite dilution activity coefficient is used to estimate the mixture properties.
Plume lengths were measured to be ∼2 km or more with a dilution rate coefficient about a factor of ten lower than commonly observed for daytime.
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