Spectra of the subsequent dilutions checked the effectiveness of the 100 × dilution steps, i.e. verified that a) the lower dilutions (from pharmaceutical factory and prepared in the laboratory) were comparable and b) the provided higher potencies were effectively diluted.
72 hours later puromycin was removed and cells were cloned by serial dilution and checked for PML expression by immunofluorescence microscopy using antibody against all PML isoforms.
The stability of the oligomeric species upon dilution was checked and the effect of dilution on the number of oligomer detected was found to be very small, just 3 times higher for type A oligomers and 1.5 times higher for type B oligomers (data not shown).
Additionally, the dilution rate was checked manually by measuring the mass of the harvest outflow within a timespan of one hour before sampling.
With that in mind, check the level of the system first, then check the dilution.
As for checking the dilution of the anti-freeze, there is a tool that will quickly measure the temperature at which the anti-freeze will actually begin to freeze.
Samples were visually checked for dilution and haemolysis.
The solvents and the dilutions employed were checked for any potential contamination of the PBDE analytes before their use.
Antifungal activities were checked through agar tube dilution method whereas potato disc assay was employed for the determination of antitumor activity.
The efficiency was estimated using series of 5-fold dilution of the sample and checked for each run.
