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Briefly, freshly thawed serum was diluted with dilution buffer and added to each well.
Samples were diluted with dilution buffer and the absorbance taken at 405 nm.
The sonicated DNA was diluted in ChIP dilution buffer.
Prior to each ligand binding assay, NTS1 was diluted into receptor dilution buffer.
Then the protein-DNA complex was diluted in ChIP dilution buffer containing protease inhibitors.
The sections were incubated overnight at 4 °C with primary antibodies diluted in antibody dilution buffer.
The sheared chromatin was diluted in ChIP dilution buffer to a final SDS concentration of 0.1%.
Samples were diluted in 1 4 dilution buffer 10 mM Tris/0.5%BSA/0.05%Tween-20Tween-20
The fecal sample was diluted in the dilution buffer supplied with the test.
The resulting chromatin was diluted 10× in ChIP dilution buffer and 1% put aside as input sample.
The kit includes concentrated assay reagent, dilution buffer, and pre-diluted DNA standards.
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