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Suitable Hb concentrations for calibration were obtained by diluting the concentrated lysate with solution A. Free calcein was added to the Hb samples from the 10 mM stock in DMSO to render the indicated final calcein concentrations.
Doses of vaccine are prepared by diluting the concentrated product with 0.9% sterile saline solution to the required concentration and volume.
For the 2D analysis the samples were desalted by diluting the concentrated proteins to 0.5 ml with 10% PBS and re-concentrating them as above.
These phantoms were prepared by diluting the concentrated Intralipid® 20% solution (manufactured by Fresenius Kabi, Uppsala, Sweden and distributed by Baxter Healthcare Corp., Deerfield, IL) in de-ionized water.
The pH was then adjusted by diluting the concentrated enzyme four times with 50 mM MES/HEPES/CHES/formate mixed buffer at pH 3.5 or 7.5 in a septum-sealed conical vial.
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We diluted the concentrated aerosol with 150 L/min of room-temperature dilution air [relative humidity (RH) ~ 30%], which was added to provide enough airflow for the human subjects' breathing requirements.
So we now diluted the concentrated wort and cooled it down.
Bratton, who is no stranger to controversy having held senior police positions at similar times of strife in Boston and Los Angeles, as well as in New York between 1994 and 1996, sought to dilute the concentrated feelings around the policing issue by casting it as a problem with wide causes.
We then diluted the concentrated samples 1 10 with the normal isoform of prion protein substrate containing 0.5% potassium dextran sulfate.
In addition, the 7 to 10% (w/v) ready-to-ferment sugars in watermelon (glucose, fructose, and sucrose) would supplement the primary feedstock and reduce primary feedstock requirements proportional to the volume of watermelon juice used to dilute the concentrated feedstock.
Fill the brining bag with 1/2 - 1 gallon of cold water to dilute the concentrated brine and cover the ham.
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