The inoculum was cultivated overnight in nutrient broth at 37 °C and serially diluted up to 10−3 dilution.
However, there were also eluates from products such as ethylene propylene diene monomer rubber (EPDM), sealing masses, and synthetic flooring that had to be diluted up to a dilution factor above 1000 to derive LID values.
Stock solutions of aqueous P60 (6.6 mg/mL) and caffeine (13.3 mg/mL) were prepared and further serially diluted (up to 6 dilutions) in the nutrient broth.
The culture was then diluted up to 50 mL (1 10 dilution) with fresh YPD liquid medium and incubated for 4 hr at 30°/230 rpm.
S05 brain material was serially diluted up to 10-fold, then each dilution was used to seed serial dgPMCAb; 18 serial dgPMCAb rounds were conducted and analyzed by Western blot.
The resultant soil solution was then serially diluted up to 10−6-fold, and the respective dilutions were plated on Martin agar medium supplemented with 30 μg/mL streptomycin, and incubated at 28 ± 2 °C for 2 3 days (Bonito et al. 2016; Mehta and Nautiyal 2001).
The FAM-labeled PCR products were appropriately diluted (up to 1/40) and 1 μl of the dilution was supplemented with 10 μl formamide (Roth, Karlsruhe, Germany) and 0.5 μl of GeneScan ROX 500 marker (Applied Biosystems, Darmstadt, Germany).
Then phage mixtures were diluted up to 10−8 in LB broth by serial ten-fold dilutions.
The digested samples were diluted up to 50 mL using nitric acid (0.5 M).
For each sample 40 μl were diluted up to 1 ml with nanopure water.
This solution was diluted up to the desired concentration to align the instrument.
