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Small volumes are normally better tolerated for preparations with known palatability issues, unless a more diluted preparation may allow better taste masking.
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The number of phagocytosed bacteria was determined by hypotonic lysis and the plating of serially diluted preparations.
Highly diluted preparations of substances that cause symptoms in healthy individuals are used to stimulate healing reactions in patients who display similar symptoms when ill [ 1].
The limit of detection for the organisms was ~1 log10CFU per mL, as determined by assessing recovery of serially diluted preparations of the test organisms applied to instruments.
Homeopathy is based on the 'principle of similars', whereby highly diluted preparations of substances that cause symptoms in healthy individuals are used to stimulate healing processes in patients who have similar symptoms when ill.
Diluted preparations obtained from Apis mellifica are reported in the homeopathic literature to have anti-inflammatory activity.
Then, 500 μl of the diluted enzyme preparation was mixed with 500 μl substrate solution (1% w/v Azo-Xylan).
A volume of 100 μl of each diluted antibody preparation was added to duplicate ELISA wells and incubated for 4 h at 37°C.
In a 20-μl assay volume 8 μl of the rPrP/antibody mixture, 2 μl compound (final concentration in primary screening: 10 μM), and 10 μl of the diluted PrPSc preparation were mixed.
Duplicate wells of Nunc Maxi-Sorb 96-well plates (Scientific Laboratory Supplies Ltd ,Nottingham, UK) were coated with 100 μl of diluted antigen preparation, using whole lung homogenate soluble fractions (10 μg total protein/ml) or the individual candidate protein antigens shown in Table 2.
To evaluate the transcriptional state of activation of each SCGB1A1 gene, end-point limiting dilution (EPLD -PCR was pEPLD -PCRwash serially diluted cDNA performedons from adult equine lung (n = 3) and uterus (n = 3) tissues.
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