Recombinant BmNPV- CP-/bx-GGT2 stock [ 15], whose titer was determined by end-point dilution method, was diluted and its baculovirus DNA was extracted from each diluted baculovirus DNA solution by High Pure Viral Nucleic Acid Kit (Roche Diagnostics K. K).
The dispersions were further diluted using a threefold dilution method in glucose stock in such a manner that the second through the seventh rows of wells contained sample dispersed in concentrations of 3.33%, 1.11%, 0.37%, 0.12%, 0.04%, and 0.01%.
Carbonate and bicarbonate analysis was carried out using acid titration (1 N diluted sulphuric acid) method; Chloride concentration was measured by AgNO3 titration method and sulphate was determined by BaCl (turbidity techniques) method using a spectrophotometer.
In such cases, an expert performing microscopic scoring used to deal with part of field of quantification restricted to hot spots, while the automatic method diluted the score by counting the number of cells from the whole area of the rectangle.
For lactic acid bacteria (LABs) surface swabs were also serially diluted following similar methods as for total bacterial count but the dilutions were pour plated on MRS (Oxoid, UK) agar then incubated at 37 ± 2 °C/48 h.
Samples of milk-handling container surface swabs and milk were serially diluted following similar methods as for total viable count, but appropriate dilutions were pour-plated on MRS agar (Oxoid, UK) then incubated at 37 °C for 48 h and typical LAB counted.
Complimentary DNA (cDNA) was synthesized and diluted according the methods described previously [47].
Membranes were blocked for 1 h in TBS, 0.1% Tween 20 and 5% non-fat dry milk, followed by an overnight incubation with primary antibodies (see Materials and Methods) diluted in the same buffer.
The GOS Graphene Laboratories Inc.., Calverton, NY, USA) was manufactured by Hummer's method and diluted in water to a concentration of 2 mg/ml.
Filtrate aliquots of curcumin solutions were quantified by a validated HPLC method and diluted to reach 1 mℳ of curcumin (Evrard et al, 2004).
