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The iSG saliva was diluted in PBS.
Samples were diluted in IC-grade water (Sigma Aldrich).
The RNA pellet was diluted in 20 µl of water.
Standard samples were diluted in plasma collected from untreated mice.
Cytokines (Peprotech, UK) were diluted in complete RPMI before administration.
Antibodies and samples were diluted in blocking reagent for ELISA.
The samples were diluted in 20 mM Tris, pH 8.
All antibodies used were diluted in PBST with 5% milk.
The plasmid was diluted in saline containing 0.05% bupivacaine.
Sera were similarly 4-fold diluted in the same buffer.
After obtaining CSF, 10 microg sufentanil diluted in 1.8 ml saline was injected.
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