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The Br and Cl concentrations of the hypothetical end-members are diluted (Figure 2c).
The predicted non-destabilizing (neutral residues) ctArx1 mutant behaved like wild-type ctArx1 and remained soluble up to 53°C (at 8 mg/ml) and up to 55°C (6-fold diluted; Figure 4B).
However, the predicted destabilizing (adaptive residues) ctArx1 mutant remained soluble only up to 49°C (8 mg/ml) and 50°C (6-fold diluted; Figure 4B; Additional file 1: Figure S3).
Similar(57)
CL3TCR+CD4+ cells displayed an 8 times higher Ag-specific responsiveness than the CL3 TCR+CD8+ ones and were still able to produce IFNγwhen CaPo13 cells were diluted 1∶500 (Figure 6A).
Importantly, once the generation of the primary signal is shut off, the system behaves analogously to our simple model: the secondary signal moves more slowly than the primary signal, contracting as the primary signal dilutes (Figure 10).
The GFRa2 ligand, Neurturin (50 ng/ml) has a trophic effect in the spheroid number when culturing in a diluted (0.5×) SpherM (Figure S3-C) indicating the GPS dependence of a functional RET/GFRa2/NTN pathway.
The modified application of the nanosols resulted in even higher mechanical strength of the mortar with almost similar increase of more than 500% compared to the untreated material: diluted nanosol (5…25 g/L) (Figure 1), and coating with hydroxypropyl cellulose gel after application of nanosol diluted with acetone (Figure 1).
Groups of five mice were sacrificed 2, 3, 4, and 7 days after infection and viable bacterial numbers were determined by plating of diluted tissue homogenates (Figure 8A, B).
First, correctly folded NTS1 suffers damage by exposure to detergent and by changes in buffer composition as seen by the loss of [3H]NT binding over time in the SN, NiE and diluted NiE fractions (Figure 2, Table 1).
The results of the F 3D CSI experiments to image the diluted PFA are shown in Figure 5. Figure 5B shows a color coded sagittal slice of the 3D data set of the 0.47% (v/v% = 3.71 × 10 6 M or 6.31 × 10 5 N fluorine) PFA solution.
Hemocytes were incubated with a custom-made antibody raised against two ApMIF1 peptides (shown in the Additional file 1: Figure S1) (diluted 1/1000 in PBS1X-0.05 PBS1X-0.050) for 1 hour at room temperature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com