Sentence examples for diluted compound from inspiring English sources
Sensitivity against cladosporin was tested by growing the stains in serially diluted compound as described previously.
Reversal of fondaparinux was measured following addition of serially diluted compound.
M2 was the only highly diluted compound that diminished B lymphocytes precursors, which produce antibodies, responsible to humoral immunity.
ATP, CCh, GABA, or 5HT were delivered by switching perfusion flow from the main AHL line to another channel containing diluted compound after 30 s of baseline recording for desired durations followed by a return to AHL flow.
Compounds were diluted in half-log series in DMSO, and 2-μl volumes of diluted compound, or vehicle to high and low controls, were added to the enzyme and incubated for 30 min at room temperature before initiation of the reaction with 5 μl of 20 mM MgCl2 and ATP at the appropriate previously determined K m value.
For PAMPA experiments, 96-well multiscreen acceptor filter plates with 0.45 μM Immobilon-P membranes (Millipore # MAIPNTR10) were coated with the polar lipid 1,2-dioleoyl sn-glycero-3-phosphocholine (Avanti # 145414) and incubated for 10 min. Test compounds were diluted to 100 μM in aqueous pH 7.4 buffer (Fisher # SB110-1), and 150 μL of diluted compound was added to each well.
The following day, 5-fold serial dilutions of the tested compounds were prepared and the diluted compounds were added in duplicate in 50 μL/well to the cells.
The cells were incubated with the serially diluted compounds or proteins for 48 h.
The culture medium was replaced with medium containing serially diluted compounds in the presence of 10% FBS and 0.5% DMSO.
For HCVcc, HCVpp or VSV-Gpp, the serially diluted compounds were mixed with a certain titer of virus and added to different cells.
Briefly, 25 μL diluted whole blood (1 200 dilutions in sterile HBSS++) or 25 μL of PMNs (1 × 106) cells were incubated with 25 μL of serially diluted compounds with concentration ranges between 3.2 50 mg/mL.
