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In periodically diluted batch culture experiments similar to the original study, we used flow cytometry to observe robust switching and bistability when inducing with either 2 mM IPTG or 500 ng/μL dox in cultures growing at 37 °C.
Diluted batch cultures were grown in sterile square 1 L Nalgene bottles as single filaments with gentle bubbling, sufficient for preventing aggregates formation without harming the integrity of the filaments.
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In our study, we have shown that all foxes infected experimentally intracranially with undiluted stock or diluted batches of EBLV-1 and 2 exhibited clinical signs of rabies and succumbed between 8 and 282 days post infection.
Another batch of CNPs (batch-2) was prepared by diluting batch-1 to 1000 times, keeping the pH constant.
For exponential growth, cells were diluted by batch culture twice a day to keep them in exponential phase.
There is speculation that, rather than destroying the contaminated heroin, dealers in Scotland may have "cut," or diluted, the batch with other supplies; this may have resulted in a reduction in the size of the inoculum.
Physiological responses of phytoplankton are typically assessed under quasi-constant environmental conditions using dilute batch or chemostat cultures.
Prior to the experiment, cells were pre-acclimated to the experimental settings for 10 days in dilute batch cultures, maintaining exponential growth.
Sepsis was induced by an intraperitoneal injection of faecal slurry (n = 10), which contained faeces collected from several animals of the same batch, diluted in Ringer's acetate (suspension 25%), and then filtered.
In bead assay, neutral Affi-Gel beads (150 300 μm mesh, Biorad) were incubated with protein batches diluted to 400 ng/μl in PBS and rinsed, then deposited onto various spots of the explant surface.
The protein concentrations in various Myogel batches were diluted using cell culture media described in the cell culture -section (see below) to match those of Matrigel®.
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