To verify antigen-binding specificity, diluted (1 400) maternal plasma samples were incubated with 400 μg of purified recombinant LDH, cypin, YBX1, CRMP1, CRMP2 or STIP1, or with buffer alone for 20 h at 4 oC.
These results, together with the high seroprevalence rates and ELISA positivity on highly diluted sera, support the specificity of the observed IgG reactivity.
To increase specificity, we diluted the PCR product from the first PCR reaction 1 10 before using it for the second PCR reaction.
The sensitivity of the antibody was tested using serially diluted RutR and its specificity was tested by spiking purified RutR into a whole lysate of E. coli.
(c) The dipstick RDT did not perform satisfactorily on diluted whole blood (sensitivity 73%; specificity 57%).
Immunocytochemical controls only included a replacement of the primary antiserum by appropriately diluted normal serum, since the specificity of the antisera was extensively tested and approved by the manufacturers.
The efficiency of the amplification reaction for each gene was calculated by running a standard curve of serially diluted cDNA sample, and the specificity of the amplification products was checked by analysis of the melting curve.
Hyperpolarized (HP) magnetic resonance imaging (MRI) is poised to revolutionize the field of molecular imaging by enabling the tomographic detection of dilute nuclear spin systems with biochemical specificity but without ionizing radiation.
To demonstrate Perp antibody specificity, Perp antibody was diluted 1 50 in PBS containing 2 μg/μl Perp C-terminal crude peptide (containing the last 20 amino acids of the Perp protein sequence [PNYEDDLLGAAKPRYFYPPA]).
For this reason, for Myc and Mad, we use the same approach as for homodimers, recognizing that the specificity signal will be diluted because of the heterodimerization with Max.
Sections were then incubated with primary monoclonal antibodies diluted in MP/PBS (LM19, LM20, LM5, LM6, LM15, LM10, LM11, LM21, www.plantprobes.net, diluted 1 10; CCRC-M1, http://www.ccrc.uga.edu/~carbosource/CSS_home.html, diluted 1 5; and BS-400-2 BS-400-2 BS-400-2pplies.com.au/, diluted 1:25) for 1 httpor specificity of probes see Table 1).
