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Data is normalized to the most dilute sample to be represented on the same scale.
If peak areas of analytes in sample extracts are above calibration curve, dilute sample extract solution with extraction solvent accordingly.
A model analyte was used to demonstrate the versatility of the injector design for low and high sample loading regimes suitable for concentrated and dilute sample solutions, respectively.
The detection limits, calculated as 3 × SD where SD is the standard deviation of the most dilute sample, were of the order of a few μg mL−1.
A 10 -μl drop from a very dilute sample solution was deposited on a carbon-copper grid and left to evaporate in air.
Total RNA was isolated from each TriPure dilute sample and each sample was resuspended with 35 µl of ultrapure water treated with 0.1% diethylpyrocarbonate (DEPC; Invitrogen, Carlsbad, CA).
Similar(42)
As the experimental data could be collected only from reasonably dilute samples, the samples were diluted five times (average 7.5 pH) before recording the data.
Thus, the flow-through sensing approach is especially advantageous for providing more reasonable sensor response times when detecting dilute samples.
Second, very dilute samples should be avoided completely (Appendix S1, section 1).
The challenge of detecting cytokines becomes larger when examining small or dilute samples, such as breath condensate [13], [14].
Sterile buffered deionised H2O was used to dilute samples.
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