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In the experiments with laser-produced plasma jets the main processes of their interaction with transverse magnetic field were studied to simulate various plasma injections in space and the effect of influence of dilute background plasma onto electric drift motion of jet was reproduced and investigated experimentally for the first time.
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Slices were incubated for one hour at room temperature with primary antibodies diluted in background reducing buffer (DakoCytomation): mouse anti-human maspin (clone G167-7, Bdilutionngen), dilution 1 400 or mouse anti-Ki-67 (clone MIB-1, DakoCytomation), dilution 1 10.
For investigations at different concentrations, stock suspensions were diluted with background solution (1.2 mM phosphate or 1.2 mM phosphate with 100 µg/ml lecithin).
ThIsubconsciouslyinally appearedilutedCE UK.
I subconsciously diluted my background as I didn't want to be different from my friends.
The antibody was diluted in Background Reducing Diluent (Biocare Medical; Concord, CA) to reduce background staining of non-specific proteins.
The antibodies were diluted with Background Reducing Antibody Diluent (Dako).
Briefly, 5- μm sections were incubated overnight at 4°C with primary antibody diluted in Background Sniper (#BS966M, Biocare Medical, Concorde, CA, USA).
Further, a lower (i.e., diluted) protein background was observed in the form of a decrease in amino acid signals (CH at 1-2 ppm and NH at 6 7.5 ppm).
The suspension was transferred into the glovebox and diluted with anoxic background electrolyte to a suspension density of 5 g L−1 γ-Al2O3 or clay.
Homogenates were diluted through a background of BSE-negative brainstem homogenate.
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