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In low-throughput experiments where we carefully count cell number at each passage and dilute back to 5 × 10 cells per milliliter every day, the dilution ranges from 1 600 for healthy cultures to 1 7 when cells are deeply senescent.
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To that end its 20% stockpile will either be diluted back down to 5% or converted into fuel rods from which re-conversion is impossible.
Cultures were diluted back to OD 0.2 0.5 then allowed to grow back to OD 1.0 at room temperature.
Exponentially growing cells in PAB medium were diluted back into fresh PAB medium with or without 1% xylose to inducce overexpression of GFP-MinC/MinD.
At each interval, the aliquot was diluted back to the initial OD600 with fresh LB.
Sample was diluted back to 2 M urea, trypsin was added, and digestion was allowed to proceed overnight at 37 °C.
The cultures were again diluted back into fresh media to a concentration of 1 × 10 cells/mL.
Thawed supernatant and pellet samples were diluted back to the original concentration with 50 mM citric acid buffer.
After growth overnight, cultures were diluted back to 0.01 OD600 and then grown in SC to approximately 0.2 OD60.2
Log phase cells were diluted back to an OD600 equal to 0.4 and grown with constant agitation in 96-well plates (NUNC) at 30 °C.
Samples were diluted back into fresh media to a concentration of 1 × 10 cells/mL and grown for 22 hr at which time cells were counted.
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