Digitised microscopic sections are displayed on monitor.
Imagescope software (version 11.1.2.760; Aperio, CA, USA) was used to view, delineate and analyse digitised tissue sections.
In contrast to static systems, virtual slides (static-dynamic hybrids) enable unrestricted examination of entire digitised tissue sections [ 5, 23- 27].
The VPS is an interactive microscope emulator that presents a complete digitised tissue section via the Internet or CD ROM, allowing both stepwise increases of magnification (16× to 2000× on standard 17" monitor) and eight-way directional lateral-motion.
Cortical damage was defined as areas of decreased neuronal density or frank cortical necrosis and evaluated on digitised brain section images using the ImageJ analysis software (U.S. National Institutes of Health, Bethesda, Maryland, USA) [ 31].
From left to right: HE-stained 20-μm section, delineated infarct area (arrow, contour), digitised autoradiogram of the same section where focal uptake matches infarct area.
Digitised autoradiogram of 20-μm sections of the spleen (positive control) and muscle (negative control), with the same colour scale.
After identifying preneoplastic and preinvasive lesions by light microscopy, we determined the DNA content of their nuclei in Feulgen-stained sections using a high-performance digitised image analyser.
All tissue sections assessed visually were submitted to digitised image analysis to measure the mean optical density (OD) of staining.
All sections from each pancreatic rudiment were digitised using cooled, three-charge-coupled-device cameras (Hamamatsu, Middlesex, NJ, USA) linked to a fluorescence microscope (Leitz DMRB; Leica, Rueil-Malmaison, France).
