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In order to dissect the same AOIs using both methods, the post manual dissected tissue sections were digitally outlined and used as guides for mesodissection.
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Captured images were digitally processed using multiple parameters outlined by the operator and presented in a software module that enables selection of cells of interest.
But with many users of such services not necessarily digitally-savvy, the budget has outlined a new standard of usability that services must adhere to in order to make sure some citizens won't be left behind.
To normalise for protein concentration, membranes were stripped using Restore Western Stripping Reagent (Fisher Scientific, Loughborough, UK) and reprobed for β-tubulin as outlined in Table 2. Images were digitally captured, band intensities were quantified using densitometric analysis using the GeneTools image analysis software (Syngene, Cambridge, UK) and normalised for β-tubulin expression.
All sections were digitally scanned and specific lesions and regions of interest were manually outlined, and lesional activity was defined as described above.
Andy Dunn wrote a terrific post outlining The Rise of Digitally Native Vertical Brands, but how does one know which "DNVBs" have the highest chance of success?
The union is battling the producers over a contract that, in addition to setting base pay rates for actors in filmed productions, would outline pay scales for work transmitted digitally over the Internet and by other means.
Using Photoshop, he digitally altered the mountain's outlines so that they traced trajectories of nine market movements over time, in a series he calls "High Altitude".
While rival companies run many identical colour copies off one original painted card, Kovacheva digitally designs and prints the outline, then hand paints each card in watercolour before hand finishing with stones, glitter and trinkets.
Both of these techniques lead to characters having white outlines when copied digitally onto the scene.
These outlines were then digitally transferred to photomicrographs from c-fos stained sections using visual landmarks as a guide for transfer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com