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The study models were used to measure the pre−/−post surgical width of the anterior and posterior dental arches with a digital sliding caliper.
The orthodontic examination horizontal overjet (distance between upper and lower incisors) was determined by using an orthodontic digital sliding calliper.
Both mandibular protrusion and mouth opening (including the vertical overbite) imposed by the oral appliance were measured with a digital sliding calliper.
The overjet (sagittal distance between upper and lower incisors) [ 14] was measured using an electronic digital sliding calliper (JOCAL, C.E. Johansson, Eskilstuna, Sweden).
Models were also placed and rimmed into maximal occlusion in order to perform measurements with a digital sliding calliper with a 0.01-mm resolution [ 26].
In order to provide for reliability and consistency in tooth width measurements, one of the investigators (WB) did all the measurements on the dental casts with a pair of digital sliding vernier calipers (Mitutoyo, Southampton, U.K) in natural light as described by Hixon and Oldfather [ 11].
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Slides were digitalized (Mirax Desk Digital Slide Scanner, Zeiss, Göttingen, Germany) and macrophages were counted and normalized for analyzed section area (Mirax Viewer, Zeiss).
Stained sections were scanned and digitalized using an Aperio ScanScope digital slide scanner (Scanscope Console v8.2.0.1263, Aperio Technologies, Inc., Vista, CA).
Conventional immunohistochemical slides were digitalized for image analysis using a digital slide scanner (Aperio Technologies Inc., Vista, CA, USA).
Images of 3, 3′-diaminobenzidine stained sections were digitized with the Pannoramic 250 Flash II digital slide scanner (3DHistech, Budapest, Hungary), and the images were captured with the Pannoramic Viewer software.
7. DIGITAL SLIDE SHOWS No more carrying that bulky slide carousel.
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