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Functional vessel density, diameter of microvessels and red blood cell velocity were visualised by both orthogonal polarisation spectral™ imaging and fluorescence microscopy and analysed using a digital image system.
They were stored at 4°C and protected from light for ∼24 h before examination and imaging on a fluorescence microscope with a MacProbe v4.3 digital image system.
A video digital image system and thermochromic liquid crystals were used to reproduce the bubble movement as well as the wake drift of the liquid.
Follow up information was also acquired from review of the medical records and archived digital image system.
A digital image system was used to determine the density of the bands (Bio-Rad, Hercules, CA).
Proteins on the membrane were detected using a West pico chemiluminescence detection kit and quantified with a ChemiDoc (BioRad) digital image system using a QuantityOne software.
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The images were processed and cross-sections were compared by means of the MIP-4 image analyser software (Digital Image Systems, SL, Barcelona) in order to quantify the bone fraction.
Briefly, a charge coupled device (CCD) camera (either a 16-bit Cascade 512F or a 12-bit Quantix 0206, Photometrics, Tucson, AZ, USA) equipped with an inverted 50 mm AF Nikon lens (1∶1∶8, Meliville, NY, USA) combined with an extender (model PK-13, Nikon, Meliville, NY, USA) was used for imaging and controlled by V++ Precision Digital Imaging System software (Digital Optics, Auckland, NZ).
For 182 patients, photos taken with the EyeGo system were compared to images taken by existing clinic cameras: a BX 900 slit-lamp with a Canon EOS 40D Digital Camera and an FF 450 plus Fundus Camera with VISUPAC™ Digital Imaging System.
The gel was stained with ethidium bromide (0.5 μg/ml) followed by visualizing the different forms of DNA (Diana III Digital Imaging System, Raytest, Straubenhardt, Germany).
Whole-core photographs of the "archive-halves" were taken on film during core description in May 2005, and subsequently line-scan digital images from the film at 3000 dpi were captured with the Digital Imaging System (http://iodp.tamu.edu/database/coreimages.html).html
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