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The gene- and exon-level digital expression is inferred by the approach presented in the Methods section.
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I think new forms of digital expression are interesting and laudable — I'm a big fan of Snapchat's experimentation with the medium and wish others would take risks like it has.
Digital expression was obtained using RNA samples from organs at different development stages and under different stress conditions.
Read mapping, read normalization and global digital expression were carried out by the bioinformatics department of Sick-Kids Hospital, Next Generation Sequencing service (Vancouver, Canada).
We next examined whether digital Myc expression is common to both CD4+ and CD8+ T cells.
In order to understand the impact of JA and MeJA on L. gmelinii at the transcriptional-level, the analysis of differentially expressed genes (DEGs) using digital gene expression was conducted.
Note: Digital expression data is shown as reads per million and qRT-PCR results were shown as relative fold changes compared to 10 DAP Our digital expression analysis identified a total of 3,023 differentially expressed genes with at least two-fold difference in expression levels during watermelon fruit development and a false discovery rate (FDR) < 0.01 (Additional file 3).
Digital gene expression was assessed using DESeq.
Differential digital gene expression was deemed with FDR value ≤0.001 and |log2 Ratio| ≥ 1 in sequence counts across libraries.
Digital gene Expression was carried out by DEGseq package in R-bioconductor 2.15 for each library with reference to control.
Digital gene expression was performed to find the tissue-specific gene expression, and correspondence analysis was executed to identify common and specific gene expression by selecting four biotic stress-related libraries.
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