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It'll incorporate secure certificate signing for identity authentication, as well, but Wacom is positioning it as a new standard for the digital expression of human creativity.
The goal of this paper is not to evaluate the digital expression of individual genes; instead we are looking at the tissue enriched digital expression level for a given chromosomal region.
Indeed, with a typical sequencing depth, we were able to obtain digital expression measures for many low-abundance genes, for which expression microarrays do not have sufficient sensitivity (Additional File 2).
The 'metabolic pathways' contributed to the greatest parts (3,473 members, 24.62%), followed by 'spliceosome' (1,320 members, 9.36%) and 'plant-pathogen interaction' (1,180 members, 8.37%) (Table 2; Additional file 2).> To generate digital expression signatures for Anthurium plants under low temperature, we used Solexa (Illumina) technology for sequencing.
This dataset allowed construction of a stage-specific digital expression profile for many transcripts [31].
Our digital expression analysis for the time course of glucose deprivation by next-generation sequencing showed that the expression level of Serum and Glucocorticoid-regulated kinase (Sgk1), which inhibits PMM2 enzyme activity [20], was up-regulated tenfold by glucose deprivation (data in preparation).
qPCR validated the digital expression data for a selection of 23 genes with high or low expression levels.
This enabled the allocation of a tentative digital expression profile for each transcript-derived contig (Additional file 4).
The total reads coverage per gene was used as digital expression value for the differential expression analysis, performed separately on 454 and MiSeq data.
We measured the raw digital expression counts for each gene by quantifying the number of EST reads that were mapped to each reference sequence.
Finally, can we re-use the mRNA-Seq data to create a tissue and organ-specific digital expression profile for each assembled contig?
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Justyna Jupowicz-Kozak
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