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This suggests that our digital counting scheme based on the parameters presented here is able to quantify the absolute copy number of nucleic acid molecules before PCR amplification.
(a) Scheme of digital counting.
Required number of random bases for digital counting.
By deep-transcript digital counting, 80% of H3K4me3 and 36% of comodified promoters were found to be transcribed.
As expected, the removal of substitution errors is not enough to make the digital counting system work.
(b) The first requirement for accurate digital counting: each target molecule should be labeled by different barcodes.
Similar(26)
When voting is finished, boxes in 54 percent of precincts are opened and the votes recorded on the vouchers are counted to verify the digital count.
Under election law, when voting is finished, an audit is automatically conducted in 54 percent of the precincts, where the boxes are opened and the votes recorded on the vouchers are counted to verify the digital count.
The resulting mean digital count difference between the reflectance standards was used to convert digital counts into reflectance units.
All data are recorded in units of digital counts (Fig. 5).
The digital counts of the first two areas are used for data processing at DE, and the counts of the last area are used for smear correction at the ground data processing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com