Exact(6)
Histomorphometric analysis was done by digital analysis system (OsteoMeasure; OsteoMetrics).
After staining cells were analysed using a Quantimet 600S digital analysis system (Leica Microsystems, Rijswijk, The Netherlands).
Bone histomorphometry was performed using a microscope (Nikon, Tokyo, Japan) equipped with a video camera and digital analysis system (OsteoMeasure; OsteoMetrics, Decatur, GA, USA).
After 6 and 24 h, the wells were photographed with CCD optics and a digital analysis system (Image Pro Plus), respectively.
The amplified products were separated by electrophoresis on a 10% polyacrylamide gel, stained with SYBR Green (Lonza, Rockland, ME), and photographed with the Kodak EDS 120 Digital Analysis System.
After 6 h of incubation at 37°C, the formation of capillary-like networks was examined under an inverted microscope (Zeiss, Oberkochen, Germany), equipped with charge-coupled device optics and a digital analysis system, and quantified using the Angiogenesis Analyzer toolset (26) for National Institutes of Health ImageJ software.
Similar(54)
Hybridization signals were evaluated using a digital image analysis system based on an epifluorescence (Provis AX70, Olympus, Milan, Italy), and images were acquired with a digital CCD camera C4742 Orca II (Hamamatsu, Japan) driven by Cytovision (Applied Imaging Corp., Santa Clara, CA, USA).
By means of a digital image analysis system, homologue points are found on the two micrographs.
Subsequently, the fibers are segmented with a digital image analysis system and the length of the segmented fibers is determined.
The analysis was based on historical maps and aerial photographs dating from 1852 to 2011 using the Digital Shoreline Analysis System (DSAS) 4.3, extension of ESRI's ArcInfo 10.×.
The net shoreline movement and end point rate statistics were generated by ArcGIS together with Digital Shoreline Analysis System software extension.
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