Immunostained cells were mounted under coverslips in Aquamount (BDH, Poole, UK) and (except where stated,) photographed using a Zeiss Axioplan microscope with an ×40 objective (numerical aperture 0.75) and Axiovision 4.6 digital acquisition software (Carl Zeiss, Oberkochen, Germany).
The output of the potentiostat was connected to an analogue/digital converter (Experiment 1: Powerlab 8/30, AD Instruments, Oxon, UK; Experiment 2: Micro 1401; CED) and then onto a PC running digital acquisition software (Experiment 1: Chart v5, AD Instruments; Experiment 2: Spike2; CED).
The HRTEM images were directly recorded on a Gatan 2K CCD camera with Digital Micrograph acquisition software.
Thin sections were stained with aqueous uranyl acetate 2% and Reynolds lead citrate and imaged in a Philips CM 100 transmission electron microscope (FEI, Eindhoven, Netherlands) using a Gatan Orius CCD camera and digital micrograph acquisition software (Gatan GmbH, Munich, Germany).
Ultrathin sections, stained with 2%% aqueous uranyl acetate and Reynolds lead citrate were imaged using a Phillips CM 100 transmission electron microscope (FEI, Eindhoven, The Netherlands) equipped with a Gatan Orius CCD camera and Digital Micrograph acquisition software (Gatan).
EM preparations were viewed and acquired at 80 kV in a 120 kV Tecnai electron microscope (FEI), using a charged-coupled device camera (1000P, Gatan) and The Digital Micrograph acquisition software (Gatan).
Image acquisition of serial sections was performed at a pixel size of 3.72 nm on a FEI TECNAI Spirit transmission electron microscope (FEI, Hillsboro, Oregon) equipped with an UltraScan 4000 4 × 4k digital camera using the image acquisition software Digital Micrograph (Gatan, Pleasanton, CA).
Photographs were made using Olympus BX41 clinical microscope and Olympus DP20 digital camera using cell acquisition software.
The images were captured by an OLYMPUS BX 51 fluorescence microscope (Center Valley, PA, USA), which is fitted with a triple DAPI/FITC/TRITC cube, a DP70 digital camera and image acquisition software.
Image acquisition was done using the Nikon digital camera DXM1200 and the acquisition software Nikon ACT-1.
Images were acquired in bright field using a Nikon DMX 1200 digital camera and ACT-1 acquisition software (Nikon Corporation, Tokyo, Japan).
