Sentence examples for digestion single from inspiring English sources

Following digestion, single cell suspensions of heart infiltrate were isolated by standard procedures and cells were stained for flow cytometry.

After trypsin digestion, single cell suspension was prepared by mechanical dissociation.

We compared the in silico digested DNA fragments (80 160 bp) by MspI and TaqαI double digestion and MspI single digestion (single digestion is from the original RRBS protocol).

In this case, Methylation Independent PCR (MIP) is firstly performed and information on the methylation status of that region is obtained through post-PCR analyses techniques like bisulfite sequencing, restriction digestion, single-strand conformation analysis, and high-resolution melting [ 6].

Additionally, results in this study are strengthened by other studies which also found that co-digesting enhanced biogas production compared to digestion of single material such as high-strength COD substrate[10, 13, 14].

The present work demonstrates the feasibility of enzymatic digestion of single DNA molecules attached to nanoparticle-modified surfaces.

If the digestion to single amino acids was not complete, carboxypeptidase Y (Sigma-Aldrich) was added to reconstituted samples for 4 hr and the analysis was repeated.

Histone samples were prepared for mass spectrometry by addition of ammonium bicarbonate to 5 mM final concentration before digestion to single amino acids by protease step-digestion as previously described.

A mixture of propidium iodide and 4′-6-diamidino-2-phenylindole was applied simultaneously with RNAse after methanol fixation and protease digestion of single cell suspensions (Volm et al, 1988b).

To derive a HCC cell line from the PBP/MED1 expressing HCC serially transplanted in athymic nude mice, PBP/MED1fl/fl tumor tissue was dispersed with collagenase or trypsin digestion into single cells or small cell clumps and these were cultured in fresh Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum.

This method is based on the specific digestion of single-stranded DNA or single-stranded regions of double-stranded DNA allowing the linearization of plasmids and resolution in PFGE as detectable bands on a faint genomic background [59].