Sentence examples for digestion gel from inspiring English sources

An internal 280bp fragment in the cloned product was excised from pBluescript SK+ by Cfr10I and BstE II digestion, gel purified, and cloned into WT cDNA replacing WT sequences.

For protein digestion gel bands were washed twice with 25 mM NH4HCO3/50% acetonitrile (VWR), followed by reduction with 10 mM dithiothreitol (BioRad) for 30 min at 56°C, carboxyamidomethylation with 50 mM iodoacetamide (BioRad) for 30 min at room temperature and subjected to digestion with trypsin (5 ng/ul, Promega, Madison, WI, USA) overnight at 37°C.

The modified BAC insert was released by NotI digestion, gel purified and used for pronuclear injection.

Prior to trypsin digestion, gel spots were washed with 25 mM Ambic and dehydrated with acetonitrile.

DNA concentrations were estimated based on fragment intensity after plasmid digestion, gel electrophoresis, and imaging using ethidium bromide.

The transgenic fragment was obtained by BsrBI digestion, gel purified followed by β agarase digestion (NEB), filtration and concentration.

The Rhpa adaptor of the HpaII-amplicon from the corresponding sample of normal tissue was removed by MspI digestion, gel-purified (Gel Extraction Kit; Qiagen, Hilden, Germany), and switched to JHpa adaptor.

Plasmids encoding full-length TALENs were linearized by NotI digestion, gel-purified (Gel Extraction kit, Qiagen), and used as templates for in vitro transcription using mMESSAGE mMACHINE Sp6 Kit (AM1340, Life Technologies).

Prior to in-gel digestion, the gel pieces were washed and dried as above.

A 5 μl of aliquot of digested DNA was assessed for completeness of digestion using gel electrophoresis.

These mitochondrial DNA mutations are usually detected by conventional polymerase chain reaction followed by restriction enzyme digestion and gel electrophoresis.