Because b-glucan is not the only ligand of dectin-1 [38], [38], we further investigated the role of b-glucan surface exposure by specific enzymatic digestion of b-glucan in the fungal cell walls using b-glucanase.
Enzymatic digestion of b-glucan in the hyphae of Aspergillus and Rhizopus was performed by using b-1-3-D-glucanase from Helix pomatia (Sigma).
The fact that Tony Smith managed to ensconce himself at an event that clearly didn't have a conceptual category suggests that they were beguiled by his triumph at Hampton Court last year where his garden "In Digestion" won Best in Show in the conceptual category.
Additionally, IL-23 production by human DCs infected with the Δags1 mutant was inhibited following blocking of dectin-1 receptor or enzymatic digestion of b-glucan (Figure 4B).
Microwave digestion followed by B and F elemental analyses, along with thermogravimetric analyses provide compositional x and δ parameters for the GICs obtained.
Viable schistosome eggs were obtained from infected mouse livers by digestion with collagenase B [ 57].
After a 30-minute digestion with cathepsin B at pH 5, 96% of pWT, 48% of pK23R, but only 2.4% of pD22G were hydrolysed.
Myocardial fragments were incubated with digestion solution (collagenase B 0.1%, dispase II 2.4 U/ml, 2.5 mM CaCl2, 138 mM NaCl, 5.56 mM glucose, 5.4 mM KCl, 4.16 mM NaHCO3) at 37°C for 30 min with frequent pipetting to ensure complete digestion.
Furthermore, the results obtained after digestion with carboxypeptidase B showed also a comparable content of basic, acidic, and main isoforms among the two products, with a main relative content of 16.4%, 30.6%, and 53.0% (n = 3) for the reference product and 8.8%, 37.8%, and 53.4% (n = 3) for Trastuzumab-Probiomed, respectively.
